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Histologic and morphologic evaluation of regenerated bladder tissue in sham and experimental groups. (A) Hematoxylin and eosin and Masson staining indicated normal bladder tissue in sham group. (B) Regenerated bladder tissue in ASC-free scaffold at 6 W. (C) Regenerated bladder tissue in ASC-free scaffold at 12 W. (D) Regenerated bladder tissue in ASC scaffold at 12 W. Scale bars = 100 μm. UE: <t>urothelium;</t> SM: smooth muscle; PLLA: poly(l-lactic) acid; SIS: small intestinal submucosa. ASC: adipose-derived stem cell; PLLA: poly(l-lactic) acid; SIS: small intestinal submucosa.
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Histologic and morphologic evaluation of regenerated bladder tissue in sham and experimental groups. (A) Hematoxylin and eosin and Masson staining indicated normal bladder tissue in sham group. (B) Regenerated bladder tissue in ASC-free scaffold at 6 W. (C) Regenerated bladder tissue in ASC-free scaffold at 12 W. (D) Regenerated bladder tissue in ASC scaffold at 12 W. Scale bars = 100 μm. UE: urothelium; SM: smooth muscle; PLLA: poly(l-lactic) acid; SIS: small intestinal submucosa. ASC: adipose-derived stem cell; PLLA: poly(l-lactic) acid; SIS: small intestinal submucosa.

Journal: International Journal of Surgery (London, England)

Article Title: Comparative study of bladder augmentation using different biomimetic scaffolds: electrospun nanofiber vs. extracellular matrix scaffold with adipose-derived stem cells

doi: 10.1097/JS9.0000000000003152

Figure Lengend Snippet: Histologic and morphologic evaluation of regenerated bladder tissue in sham and experimental groups. (A) Hematoxylin and eosin and Masson staining indicated normal bladder tissue in sham group. (B) Regenerated bladder tissue in ASC-free scaffold at 6 W. (C) Regenerated bladder tissue in ASC-free scaffold at 12 W. (D) Regenerated bladder tissue in ASC scaffold at 12 W. Scale bars = 100 μm. UE: urothelium; SM: smooth muscle; PLLA: poly(l-lactic) acid; SIS: small intestinal submucosa. ASC: adipose-derived stem cell; PLLA: poly(l-lactic) acid; SIS: small intestinal submucosa.

Article Snippet: Bladder regeneration was evaluated using immunofluorescence (IF) analysis with different antibodies against the urothelium (pan-cytokeratin antibody (AE1/AE3), Abcam, ab9377), smooth muscle (α-smooth muscle actin (α-SMA), Proteintech, 14395-1-AP), and vascular endothelium (cluster of differentiation 31 (CD31), Abcam, ab199012), and all sections were incubated with the secondary antibodies and DAPI.

Techniques: Staining, Derivative Assay

Immunofluorescence staining and quantitative assessments of regenerated bladder tissue. (A) Expression of urothelial-associated markers (AE1/AE3), smooth muscle contractile markers (α-SMA), and blood vessel endothelial marker (CD31) in regenerated bladder tissue. (B–D) Quantitative analyses of AE1/AE3 + epithelium (B), α-SMA + smooth muscle (C), and CD31 + vessel numbers (D) in regenerated bladder tissue at 6 and 12 weeks. Scale bars = 100 μm. Data are expressed as mean ± standard deviation (SD), n = 5. * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the sham group; # P < 0.05, ## P < 0.01, ### P < 0.001, compared with ASC-free at 6 weeks. AE1/AE3: pan-cytokeratin antibody; α-SMA: α-smooth muscle actin; CD31: cluster of differentiation 31; UE: urothelium; SM: smooth muscle; V: vessels.

Journal: International Journal of Surgery (London, England)

Article Title: Comparative study of bladder augmentation using different biomimetic scaffolds: electrospun nanofiber vs. extracellular matrix scaffold with adipose-derived stem cells

doi: 10.1097/JS9.0000000000003152

Figure Lengend Snippet: Immunofluorescence staining and quantitative assessments of regenerated bladder tissue. (A) Expression of urothelial-associated markers (AE1/AE3), smooth muscle contractile markers (α-SMA), and blood vessel endothelial marker (CD31) in regenerated bladder tissue. (B–D) Quantitative analyses of AE1/AE3 + epithelium (B), α-SMA + smooth muscle (C), and CD31 + vessel numbers (D) in regenerated bladder tissue at 6 and 12 weeks. Scale bars = 100 μm. Data are expressed as mean ± standard deviation (SD), n = 5. * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the sham group; # P < 0.05, ## P < 0.01, ### P < 0.001, compared with ASC-free at 6 weeks. AE1/AE3: pan-cytokeratin antibody; α-SMA: α-smooth muscle actin; CD31: cluster of differentiation 31; UE: urothelium; SM: smooth muscle; V: vessels.

Article Snippet: Bladder regeneration was evaluated using immunofluorescence (IF) analysis with different antibodies against the urothelium (pan-cytokeratin antibody (AE1/AE3), Abcam, ab9377), smooth muscle (α-smooth muscle actin (α-SMA), Proteintech, 14395-1-AP), and vascular endothelium (cluster of differentiation 31 (CD31), Abcam, ab199012), and all sections were incubated with the secondary antibodies and DAPI.

Techniques: Immunofluorescence, Staining, Expressing, Marker, Standard Deviation